Get in Touch
Blog
  1. Home >
  2. Blog >
  3. Blog Detail

High gradient magnetic cell separation with macs

Cells stained sequentially with biotinylated antibodies, fluorochrome-conjugated avidin, and superparamagnetic biotinylated-microparticles (about 100 nm diameter) are separated on high gradient magnetic (HGM) columns. Unlabelled cells pass through the column, while labelled cells are retained. The retained cells can be easily eluted

Products Recommended

  • (PDF) High gradient magnetic cell separation with
    (PDF) High gradient magnetic cell separation with

    High gradient magnetic cell separation with MACS. A flexible, fast and simple magnetic cell sorting system for separation of large numbers of cells according to specific cell surface markers was developed and tested. Cells stained sequentially with biotinylated antibodies, fluorochrome-conjugated avidin, and superparamagnetic biotinylated

  • Small but mighty: How the MACS®‐technology based on
    Small but mighty: How the MACS®‐technology based on

    Jun 25, 2010 Exactly 20 years ago, the concept of MACS high-gradient magnetic cell separation was published originally in Cytometry . To date, this article has been cited 808 times and more than 12,000 studies have been published using this technology. The diversity of cell subsets that can be addressed by the MACS-technology has been summarized in Table 1

  • Low-Resource Nucleic Acid Extraction Method
    Low-Resource Nucleic Acid Extraction Method

    immunospecific magnetic particle separation system22 to develop magnetic activated cell sorting (MACS) in the 1990s.23 These reusable columns were first composed of steel wool,23,24 which was then followed by stacked magnetic spheres with samples passing between them.25 Miltenyi’s MACS is the most prevalent use of HGMS in biological systems

  • Development of the “Three-step MACS”: a Novel
    Development of the “Three-step MACS”: a Novel

    Busch J, Huber P, Pfluger E, et al. Enrichment of fetal cells from maternal blood by high gradient magnetic cell sorting (double MACS) for PCR-based genetic analysis. Prenat Diagn 1994; 14:1129–1140 [Google Scholar]

  • Magnetic Cell Separators | Products | Miltenyi Biotec
    Magnetic Cell Separators | Products | Miltenyi Biotec

    Our manual cell separators are designed for fast and gentle cell separation when used with MACS Technology. The cell separators contain powerful permanent magnets that induce a high-gradient magnetic field within MACS Columns – a field strong enough to retain large cell numbers labeled even minimally with MACS MicroBeads

  • Magnetic activated cell sorting (MACS) pipette tip for
    Magnetic activated cell sorting (MACS) pipette tip for

    Nov 01, 2018 Magnetic activated cell sorting (MACS) pipette tip composed of nickel-wire meshes. • High-throughput and -gradient magnetic separation via simple pipetting. • High-recovery separation of bacteria contaminated in whole blood. • Successful culture and molecular analysis of sorted bacteria without off-tip processing

  • Isolation and characterization of CD34+
    Isolation and characterization of CD34+

    CD34+ cells, present at a frequency of 0.18 +/- 0.052% among leukocytes from peripheral blood (PB), can be rapidly and efficiently (recovery of 39.0-74.0%) enriched to a frequency of 38.6-87.1% (54.4 +/- 12.3%) by high-gradient magnetic cell separation (MACS) for immunophenotyping, characterization in colony-forming cell (CFU) assays, and further purification to homogeneity ( 98%) by

  • Microfluidic high gradient magnetic cell separation
    Microfluidic high gradient magnetic cell separation

    rare cells. Once the magnetic beads are bound to the cells, a magnetic field gradient is all that is required to separate them from the bulk. The magnetic beads, ranging in size from 10 nm to 10 m, are typically a mixture of polymer and iron oxide particles, Fe 2O 3 and Fe 3O 4. The separation of red blood cells from whole blood by native

  • High Gradient Magnetic Cell Sorting | SpringerLink
    High Gradient Magnetic Cell Sorting | SpringerLink

    Here we focus on applications of magnetic cell sorting with the MACS technology (Miltenyi et al., 1990, Radbruch et al., 1994, Kantor et al., 1997) which makes use of small super-paramagnetic particles and high gradient magnetic fields

  • SuperMACS™ II Separator | Separators | MACS Cell
    SuperMACS™ II Separator | Separators | MACS Cell

    The SuperMACS II Separators contains a powerful permanent magnet that induces a high-gradient magnetic field within MACS Columns – a field strong enough to retain cells labeled with even small amounts of MACS MicroBeads. . The malaria parasites digest red blood cell hemoglobin, resulting in the formation of hemozoin

  • D Columns | Columns | MACS Cell Separation | Products
    D Columns | Columns | MACS Cell Separation | Products

    Separator, the fibers amplify the magnetic field, thus inducing a high gradient within the column. This is crucial for isolation of cells which are only minimally labeled with MACS MicroBeads, leaving enough epitopes free for concurrent antibody staining

  • Purification of Pig Muscle Stem Cells Using Magnetic
    Purification of Pig Muscle Stem Cells Using Magnetic

    Sep 01, 2020 Purification of pig muscle stem cells by magnetic-activated cell sorting (MACS) The CD29-positive pig muscle stem cells were sorted using a MACS Cell Separation System (Miltenyi Biotec, Bergisch Gladbach, Germany) to remove the nonmyogenic cells during the in vitro culture. When the cells reached approximately 90% confluency, the cultured cells

  • MAGNETIC-ACTIVATED CELL SORTING - Encyclopedia
    MAGNETIC-ACTIVATED CELL SORTING - Encyclopedia

    The column consists of steel wool which increases the magnetic field gradient to maximize separation efficiency when the column is placed between the permanent magnets. Magnetic-activated cell sorting is a commonly used method in areas like immunology, cancer research, neuroscience, and stem cell

  • Magnetic particle separation, a short review - Elveflow
    Magnetic particle separation, a short review - Elveflow

    Commercial magnetic particle separation. One of the earliest works in magnetic capturing using bulk magnets was reported by Miltenyi in [1]. In this work, a Magnetic Cell Sorter (MACS) from Miltenyi Biotec is used to separate cells labeled with magnetic particles from non-labeled cells. Three basic steps can be observed: the objects of interest

  • Magnetic cell sorting
    Magnetic cell sorting

    Dear (MAC) sorters, please have a look again at the first publications describing the MACS System: Miltenyi, S., Muller, W., Weichel, W. & Radbruch, A. High gradient magnetic cell separation with MACS. Cytometry, 231-238 (1990). Even in those early days recoveries of 90% were obtained

  • Isolation and characterization of CD34+ hematopoietic stem
    Isolation and characterization of CD34+ hematopoietic stem

    CD34 + cells, present at a frequency of 0.18 0.052% among leukocytes from peripheral blood (PB), can be rapidly and efficiently (recovery of 39.0–74.0%) enriched to a frequency of 38.6–87.1% (54.4 12.3%) by high-gradient magnetic cell separation (MACS) for immunophenotyping, characterization in colony-forming cell (CFU) assays, and further purification to homogeneity ( 98%) by

Related Blog