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Advantages of magnetic bead method for cd34 separation

Positive selection of CD34+ cells has applications in diagnostic pathology, in peripheral blood and bone marrow transplantation, and in studies on the function and regulation of primitive haemopoietic stem cells. Antibody-coated magnetic microspheres (dynabeads) can be used to isolate these cells by positive selection procedures

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    Jun 01, 1997 Adaptation of this approach to harvest CD34+ cells requires a method for detaching the tar- get cells from the beads in viable form.14 After many improvements in the design of the separation systemis and operating conditions, a clinical-scale device has been developed and evaluated for stem cell enrichment: the magnetic cell separator

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    The cell mixture was sensitized with anti-CD34 (9C5) antibodies and sheep anti-mouse IgG1 paramagnetic microspheres and enriched for CD34+ cells using an Isolex 50 magnetic separator

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    Typical EasySep™ Human Cord Blood CD34 Positive Selection III Isolation Profile. Starting with fresh cord blood, the CD34+ cell content of the isolated fraction is typically 87 12% (mean SD, n=10, data obtained using the purple EasySep™ Magnet). The CD34+ cell content of the starting sample is typically 0.5 0.25% (mean SD)

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    Oct 30, 2017 CD34 + hematopoietic stem and progenitor cells are used to promote bone marrow reconstitution following cancer treatment and may offer a novel treatment for other indications. Leukapheresis of mobilized peripheral blood (mPB) is a common source of CD34 + cells. Depending on the application, it may be desired to purify the CD34 sup + /sup cells in the leukapheresis product

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    Positive selection of CD34+ cells has applications in diagnostic pathology, in peripheral blood and bone marrow transplantation, and in studies on the function and regulation of primitive haemopoietic stem cells. Antibody-coated magnetic microspheres (dynabeads) can be used to isolate these cells by positive selection procedures

  • Methods of CD34+ cell separation: Comparative
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    Jun 01, 1997 Adaptation of this approach to harvest CD34+ cells requires a method for detaching the tar- get cells from the beads in viable form.14 After many improvements in the design of the separation systemis and operating conditions, a clinical-scale device has been developed and evaluated for stem cell enrichment: the magnetic cell separator

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    A comparison of the ungated forward scatter versus side scatter profile of the same sample after separation with the CD34 MicroBead Kit (# 130-046-702) or the CD34 MicroBead Kit UltraPure (# 130-100-453) demonstrates reduced debris carry-over with the CD34 MicroBead Kit UltraPure. ... We use these CD34 immuno magnetic beads to purify our T cell

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  • EasySep™ Human CD34 Positive Selection Kit II
    EasySep™ Human CD34 Positive Selection Kit II

    Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells. This product can be used in place of the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations. Advantages